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this residue is hydrogen bonded into the lone h2o molecule (or hydroxide) that may be coordinated into the metallic ion certain to the Mβ-web-site (21). Thus, it is anticipated this residue will operate in catalysis by abstraction of the proton from water previous to, or slot naga26 concomitant with, the assault of hydroxide on the amide bond of your substrate. In the detection boundaries of our assay for solution formation, we were struggling to evaluate any catalytic activity for either the D273N or D273A mutant.

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